Detection of 8-Hydroxy-2'-deoxyguanosine in Deoxyribonucleic Acid by the ³²P-Postlabeling Method

Abstract

Using synthesized 8-hydroxy-2'-deoxyguanosine 3'-monophosphate as a marker, the ³²P-postlabeling method was adapted with minimum modifications for the analysis of 8-hydroxy-2'-deoxyguanosine (8-OH-dG) content in deoxy-ribonucleic acid (DNA). This method allows the analysis of one 8-OH-dG per 10⁴ DNA nucleotides with only 10 pmoles of nucleotides required. The amounts of 8-OH-dG in DNA detected by the postlabeling method correlated well with the electrochemical detection method but were consistently lower.