抄録
A color change of New coccine in an alimezine syrup after the mixing of commercial alimezine, lysozyme and trimetoquinol syrups was studied by means of spectroscopic methods. No color change of New coccine in the alimezine syrup was observed by an addition of either lysozyme of trimetoquinol syrup. Yet both lysozyme and trimetoquinol were required for drastic changes in absorption and circular dichroism spectra of New coccine. From these spectral changes, it was consifered that the colow change of New coccine resulted from the binding of New coccine of lysozyme. In this case, it seemes that New coccine molecules are placed in a hydrophobic lysozymal region with a specific configuration. Since the color change was observed also by a mixing of pure New coccine, lysozyne and sodium sulfite, the color change of the admixture mentioned above likely resulted from the addition of sulfite, groups to New coccine molecules. Sodium sulfite is contained in commercial syrups as a stabilizer. From the thermodynamic parameters calculated from the spectral changes, a possible role of lysozyme in the color change of New coccine was demonstrated. The values of the parameters suggest also that lysozyme fixes New coccine molecules in a specific conformation.
