Stereochemistry of Hydrogen Addition to C-25 of Desmosterol by Sterol-Δ24-Reductase of Rat Liver Homogenate

Tomoko YAGI; Noriko KOBAYASHI; Masuo MORISAKI; Noriyuki HARA; Yoshinori FUJIMOTO

doi:10.1248/cpb.42.680

Stereochemistry of Hydrogen Addition to C-25 of Desmosterol by Sterol-Δ²⁴-Reductase of Rat Liver Homogenate

Tomoko YAGI, Noriko KOBAYASHI, Masuo MORISAKI, Noriyuki HARA, Yoshinori FUJIMOTO

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Keywords: [26-¹³C]desmosterol, [27-¹³C]desmosterol, sterol-Δ²⁴-reductase, cholesterol biosynthesis, [2-¹³C][1-(methoxycarbonyl)ethyl]triphenylphosphonium iodide

JOURNAL FREE ACCESS

1994 Volume 42 Issue 3 Pages 680-682

DOI https://doi.org/10.1248/cpb.42.680

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Abstract

Chemically synthesized (26)- and (27)-methyl-¹³C-labeled desmosterol (3, 5) were separately incubated with rat liver homogenate. ¹³C-NMR analysis of the incubation products indicated that the C-25 hydrogen of cholesterol was introduced from the si-face of 3 and the re-face of 5.

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