Nitric Oxide Generation from Aromatic N-Nitrosoureas at Ambient Temperature

Abstract

Generation of nitric oxide (NO) from aromatic N-nitrosoureas 1-3 at ambient temperature without photo irradiation was confirmed by the trapping of NO as a nitrosyl complex of 5, 10, 15, 20-tetrahenylporphyrinatocobalt(II) and spectrophotometrically quantified by means of the Griess reaction using a newly designed test apparatus in which NO^-₂ is generated from NO. 3, 3-Dibenzyl-1-(4-tolyl)-1-nitrosourea (1a) showed the greatest NO-generation ability among compounds 1-3 and N-acetyl-S-nitroso-DL-penicillamine (SNAP) in chloroform. A comparison of 1a and usual NO donors (NOC-18, NOR-4, SIN-1, SNAP, and SNP) in Krebs buffer was also made using the same test apparatus.