The Catalytic activity of Glass Beads, Silica Gels and Anion-Exchange Resins Modified with Metal-Porphines in the Oxidative Reaction of NADH

Abstract

Silica gel and glass beads were modified using the acid chloride of metal-tetrakis(4-carboxyphenyl)porphine (M-TCPP) through a peptide bond, and an anion-exchange resin was modified with M-TCPP by electrostatic interaction and physical adsorption. The carriers modified with Co<3+>-TCPP proved to accelerate the following redox reaction : NADH+O₂→^^^(carriers modified with Co-TCPP)NAD⁺+H₂O₂.The formation of hydrogen peroxide was confirmed by means of a colored reaction. The glass beads medified with Co³⁺-TCPP exhibited the strongest activity among the tested carriers, and were expected to be useful practically as a solid catalyst for the formation of hydrogen peroxide from NADH.