Abstract
A cyclodextrin modified capillary zone electrophoresis (CD-CZE) for the enantiomeric separation of tetrahydroprotoberberine N-metho salts was established. The resolution was optimized by changing the concentraion of the electrolyte solution, hydroxypropyl-β-CD (0.02 M, 0.07 M, or 0.14 M) or dimethyl-β-CD (0.05 M or 0.15 M) in phosphate buffer (pH 2.5 or 3) containing 10% acetonitrile with an applied voltage of 20 kV. This method was applied toward the enantioselective bio-conversion of quanternary tetrahydroprotoberberine N-metho salts in cultured cells of Corydalis species.
