Studies on Acylase Activity and Microorganisms. IX. Enzymatic Resolution of Tryptophan by a Strain of Soil Bacteria.

Abstract

It was found that a strain of Pseudomonas sp., KT 104, isolated from the soil of a bamboo thicket in Kanazawa, has the ability to hydrolyze asymmetrically 1-N-acetyl-DL-tryptophan to give L-tryptophan and 1-N-acetyl-D-tryptophan. The strain may be used in the form of bacterial mass, acetone-dried powder, or cell-free extract. KT 104 can be cultivated in a synthetic medium containing N-benzoylanthranilic, benzoic, p-hydroxybenzoic, salicylic, anisic, or phenylacetic acid as the sole source of carbon, and ammonia as the sole source of nitrogen, at 25°, for at least 3 generations.