Determination of Fluorescence of Flavins on Paper Strips.
1958 年 6 巻 6 号 p. 659-662
詳細
1958 年 6 巻 6 号 p. 659-662
Fluorescence spectra of flavins on paper strip were determined and the peak of the fluorescence spectra of free riboflavin, FMN, and FAD was identically recorded at 530mμ. The relation between the quantity and fluorescence energy on paper strip was linear over a range of the quantities of flavin from 2.66×10-10 moles to 0.53×10-11 moles. The relative fluorescence energies of equimolar quantities of free riboflavin, FMN, and FAD on a paper strip were determined under experimental conditions reported herein. The molar quantity of flavins on a paper strip can be determined directly by fluorometry using a standard concentration of free riboflavin placed on the paper strip as a scale.
