抄録
Freezing resistance of xylem ray parenchyma cells of birch (Betula platyphylla L.) by deep supercooling increases during seasonal cold acclimation. When apoplast substances were partially eluted by an acidic solution from winter xylem tissue, freezing resistance by deep supercooling of the tissue was decreased. Several major proteins were detected in the acid-extractable fraction of xylem tissue, and the amounts of those proteins were increased in winter. Immunoblot analysis showed that some of the major proteins are similar. N-terminal amino acid sequencing revealed that they are similar to the pathogenesis-related proteins.
