低温生物工学会誌
Online ISSN : 2424-1555
Print ISSN : 1340-7902
コスギゴケ(Pogonatum inflexum)の懸濁細胞培養系確立と乾燥法による超低温保存(平成18年度 第52回低温生物工学会年会)
山崎 秀幸新井 雅雄栗山 昭
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2006 年 52 巻 2 号 p. 141-144

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Culture system of Pogonatum inlexum was established using C-1 medium containing 3% sucrose, 5×10^<-6>M 2,4-dichlorophenoxyacetic acid and 1×10^<-5>M 6-benzyl aminopurine. The cultured cells were desiccated rapidly or slowly and then cryopreserved in liquid nitrogen. Almost 100% cells survived desiccation and cryopreservation. The desiccated cells survived room temperature for 1 month. As the preculture was not needed for the desiccation and preservation, it was considered that they have high desiccation tolerance naturally.
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© 2006 低温生物工学会
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