抄録
Aristeromycin, an antibiotic isolated from Streptomyces citri-color, induced DNA synthesis and cell division of non-dividing 3T3 cells (A-31) in culture. The induction occurred in quiescent cells produced by contact inhibition or by deprivation of serum or isoleucine from culture medium. In confluent culture, the maximum stimulation of DNA synthesis was observed about 24 h after the addition of aristeromycin and was followed by cell division. The optimum concentration of aristeromycin for induction was about 0.5μg/ ml on glass dishes. In contrast to its stimulative effect on nondividing cells, aristeromycin inhibited growth when added to dividing cells. Growth of other normal cell lines in confluent culture also was induced by aristeromycin. However, growth of malignant cell lines such as SV-3T3, poly-BHK, AC and KB cells was inhibited. Adenine nucleosides reversed the inductive effect of aristeromycin.
