The TMA is an intensive and efficient method, and the technique should be used by all means in the experiment of the analytical cytometry. The good points of TMA are to save time and cost greatly for cancer research. A sample tissue for TMA is cut from a common paraffin-embedded tissue block, it is inserted in a new paraffin block (recipient-paraffin-block). Finally, a recipient-paraffin-block for TMA including many (may be more than 100) tissue samples is completed. It is thinly cut, and can be stained using antigen-antibody reaction or hybridization technique. TMA samples suit virtual microscopy system well because it has high density tissue information. To make better use of TMA, this brief review introduces outline of TMA system based on published reports and informs the limits, problems, and notes.
