Drug Delivery System
Online ISSN : 1881-2732
Print ISSN : 0913-5006
ISSN-L : 0913-5006
共焦点レーザー蛍光顕微鏡を用いたアンチセンスオリゴ核酸/脂質複合体の細胞内局在の解析
佐藤 智典西 宏高秋野 弘之岡畑 恵雄東海林 洋子嶋田 甚五郎
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1998 年 13 巻 5 号 p. 359-364

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Distribution of phosphorothioate antisense oligonucleotide (S-oligo), S-oligo/lipofectin complex and S-oligo/PEG lipid complex in Hela cells was observed by a confocal laser scanning microscope. The S-oligo localized to endosome/lysosome in a punctate fluorescence pattern. In the presence of lipofectin, the S-oligo was accumulated in the nucleus of Hela cells. The S-oligo/PEG lipid complex showed both punctate fluorescence and diffuse fluorescence in the cyoplasm, but not in the nucleus. These result clearly demonstrated that the subcellular distribution of S-oligo depended on the delivery system.
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