抄録
The present study was undertaken to examine potential value of cetlymannosides-modified multilamellar liposomes(Man-MLV)as vehicle of recombinant human macrophage colony-stimulating factor (M-CSF) to human blood monocytes. Man-MLV was taken effectively by monocytes as compared with liposomes without cetylmannoside (PC-MLV). Addition of D-mannose(50 mM) inhibited about 50% of uptake of Man-MLV by monocytes, but not significantly uptake of PC-MLV by monocytes. These results suggest that mannose redisues of Man-MLV contributes to uptake of Man-MLV by monocytes. We examined effect of encapsulation of M-CSF in Man-MLV on survival prolongation of monocytes. Blood monocytes that had been incubated for 7 days in medium with M-CSF encapsulated in Man-MLV were effective in prolongation of monocytes survival. This enhancing effect by M-CSF encapsulated in Man-MLV showed about 5-10 times higher than that in PC-MLV, in spite of the uptake of Man-MLV by monocytes was only about 2-fold different from that of PC-MLV. It was suspected that this effect contributes to not only effective uptake by monocytes, but also the difference of intracellular disposition of liposomes in monocytes. These results suggest that Man-MLV may be effective carrier vehicle in in vivo delivery of M-CSF to human blood monocytes.
