2000 年 20 巻 2 号 p. 223-231
In the clinical, growth suppression in a cleft palate patient caused by postoperative scar tissue after the palatoplasty. Recently, the metabolism pattern of the normal palatal mucosa reports a difference in comparison with the scar tissue in collagen cross-link and conformer. This fact indicates the importance of leading the collagen of the scar tissue, which exists for the palate to the normal condition, to stimulate growth of the normal maxilla. However, the research that revealed metabolism activity of the collagen included for palatal mucosa and growth of the maxilla is limited, and the elucidation is urgent. It was then made that the relevance to maxilla lateral growth and collagen metabolism existing for palatal mucosa of the normal rat was clarified as the first step of purpose in this study. The retrieval was observed by the change of the gelatinase activity, which is concerned in metabolism activity of the collagen, using histological observation by morphological evaluation and H-E staining of the rat maxillary dentition, Film in situ zymography method (the FIZ method). The following was obtained after each relevance was examined.
1. With aging, the dentition width described by the increase curve equal to the body weight. Especially, it was indicated to be the growth middle point of a 15-week-old rat nearly in puberty almost. And though the start of the growth of the dentition width was later than that of the body weight, it became a similar growth rate after 11 weeks-old rat.
2. With the aging was a narrowing of the midpalatal suture division cartilage layer.
3. Gelatinase activity of the palatal mucosa was highest at either week-old in the epithelium.The high activity was recognized in the connective tissue division in nerve and blood vessel circumference. Moreover, the activity of epiphysis circumference bone in the midpalatal suture division was highest in a 4-week-old rat, and it lowered afterward time-dependent. In an 11-week-old rat it could not be recognized.
These findings suggested that gelatinase activity, which is collagen metabolism activity, was changed with the growth of the maxilla, and it was proven that the change was obvious in the midpalatal suture.
