Silicate bioceramics elicit proliferation and odonto-genic differentiation of human dental pulp cells

Weiwei PENG; Zhiguang HUAN; Ge PEI; Jinheng LI; Ying CAO; Long JIANG; Yaqin ZHU

doi:10.4012/dmj.2021-042

Original Paper

Silicate bioceramics elicit proliferation and odonto-genic differentiation of human dental pulp cells

Weiwei PENG, Zhiguang HUAN, Ge PEI, Jinheng LI, Ying CAO, Long JIANG, Yaqin ZHU

Author information

Weiwei PENG
Department of General Dentistry, Shanghai Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology
Zhiguang HUAN
State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences
Center of Materials Science and Optoelectronics Engineering, University of Chinese Academy of Sciences
Ge PEI
State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences
College of Chemistry and Materials Science, Shanghai Normal University
Jinheng LI
Department of General Dentistry, Shanghai Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology
Ying CAO
Department of General Dentistry, Shanghai Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology
Long JIANG
Department of General Dentistry, Shanghai Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology
Yaqin ZHU
Department of General Dentistry, Shanghai Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology

Keywords: Dental pulp cells, Differentiation, Calcium silicate, Mineralization, Cytokines

JOURNAL FREE ACCESS

2022 Volume 41 Issue 1 Pages 27-36

DOI https://doi.org/10.4012/dmj.2021-042

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Abstract

This study aimed to investigate the effects of silicates on the proliferation and odontogenic differentiation of human dental pulp cells (hDPCs) in vitro. HDPCs were cultured in the presence of calcium silicate (CS) extracts, while calcium hydroxide (CH) extracts and culture medium without CH or CS were used as the control groups. The calcium and phosphorus ion concentrations in the CS were similar to those in the control groups, but the concentration of silicon ions in the CS extracts was higher than that in the control groups. HDPCs cultured with CS and CH extracts at dilution of 1/128 proliferated significantly more than those cultured with the control treatments. CS extracts promoted cell migration, enhanced the expression of odontogenic marker genes and conspicuously increased odontogenesis-related protein production and the release of cytokines, suggesting that CS bioactive ceramics possess excellent biocompatibility and bioactivity and have the potential for application as pulp-capping agents.

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