Sum Frequency Generation Confocal Microscopy Observation of a Fish Scale

Wataru Kouyama; Atsushi Ogawa; Hongyan Li; Yoshihiro Miyauchi; Goro Mizutani; Haruyuki Sano

doi:10.1380/ejssnt.2014.259

Conference -ALC13-

Sum Frequency Generation Confocal Microscopy Observation of a Fish Scale

Wataru Kouyama, Atsushi Ogawa, Hongyan Li, Yoshihiro Miyauchi, Goro Mizutani, Haruyuki Sano

著者情報

キーワード: Sum frequency generation, Microscopy, Vibrational spectroscopy, Bioimaging and engineering, Biological molecules-proteins, Fish scale, Collagen

ジャーナルフリー

2014 年 12 巻 p. 259-262

DOI https://doi.org/10.1380/ejssnt.2014.259

詳細

抄録

An optical sum frequency generation (SFG) microscopy image of a fish scale of Pagrus major was observed. Its SFG spectra were also measured and were compared with that of collagen of Achilles tendon of a cow (Bos taurus). From this comparison, the peak near 2950 cm^-1 in the fish scale spectrum was assigned to the fish collagen. The two collagen spectra showed different line shapes and widths owing to a difference in the background nonlinearity. In the SFG image of the fish scale cross section, stronger signal was observed from the sea side than from the body side. [DOI: 10.1380/ejssnt.2014.259]

責任著者(Corresponding author)

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