論文ID: 23-68128
In the present study, a progesterone (P4) biosensor was developed to detect cattle estrus. Thionin, which has been reported to oxidize steroid hormones, was immobilized on an electrode via 10-carboxy-1-decanethiol with the aim of continuous measurement of P4 in the cattle body. On the screen-printed electrode, Au nanoparticles were electrodeposited on the surface of the carbon electrode to increase surface area of electrode. Finally, the thionin-modified electrode surface was covered with Nafion™. As a result, the influence of contaminants (BSA, l-ascorbic acid) was avoided. The detection range of the prepared sensor for P4 was 1–20 nM (= nmol L−1). When bovine plasma was used as a biological sample, it was confirmed that the current response in i-t measurement increased due to the addition of P4. The fabricated biosensor was able to detect P4 for 4 days. It is expected that the P4 biosensor used in the present study will enable accurate understanding of cattle estrus.
