抄録
Various doses of parathormone were injected into rats, and calcium, inorganic phosphorus, alkaline phosphatase, α-L-glutamyl-β-naphthylamide splitting enzyme, L-leucyl-β-naphthylamide splitting enzyme, and L-leucinamide splitting enzyme were measured in serum simultaneously. Changes in these factors were dependent on the dose of parathormone.
Calcium concentration and α-L-glutamyl-β-naphthylamide splitting enzyme activity showed similar changes. Calcium concentration was increased by an injection of 300 U. S. P. parathyroid units/100g body weight, and was returned to the normal value by 600 units.α-L-Glutamyl-β-naphthylamide splitting enzyme activity was increased by 300 units of parathormone, then maintained a value slightly lower than normal by a dose from 450 to 1200 units.
Changes in the activities of L-leucyl-β-naphthylamide and L-leucinamide splitting enzyme were different. L-Leucyl-β-naphthylamide splitting enzyme activity was decreased by a dose from 300 to 900 units, and was returned to nearly the normal value by 1200 units. L-Leucinamide splitting enzyme activity was increased by a dose below 450 units, but was decreased to about half of the normal value by 1200 units. This indicates that L-leucyl-β-naphthylamide splitting enzyme is different from L-leucinamide splitting enzyme.
Serum alkaline phosphatase activity was decreased by a dose below 450 units and was maintained at 34% of the normal value by doses up to 1200 units.
