Endocrinologia Japonica
Online ISSN : 2185-6370
Print ISSN : 0013-7219
ISSN-L : 0013-7219
Effect of Hydrocortisone on Hela Cells with Special Reference to the RNA Metabolism
HIDEO SUZUKIMOTOYASU OHSAWAHIROYOSHI ENDO
著者情報
ジャーナル フリー

1969 年 16 巻 1 号 p. 121-127

詳細
抄録
Hydrocortisone (HC) inhibited the growth of HeLa cells at the concentration of 0.01-10μg/ml but enhanced it at 0.001μg/ml.
HC increased the β-glucuronidase and alkaline phosphatase activities of HeLa cells at 10μg/ml in 4 days, although it did not affect the acid phosphatase activity.
Nucleic acids obtained from growing HeLa cells were fractionated by methylated bovine serum albumin column chromatography. Two peaks of rapidly labeled RNA were found in the chromatogram, and from the experiment of time course of 32P-labeling and base composition analysis, it was assumed that one was the precursor of r-RNA and the other d RNA possibly containing m-RNA.
HC (10μg/ml) inhibited the synthesis of nucleic acid in HeLa cells; in early period of HC treatment, only the d-RNA synthesis was slightly inhibited, while with longer treatment the synthesis of r-RNA precursor was most strongly inhibited in contrast with the weak inhibition of DNA and d-RNA syntheses.
From these data, the relationship between the catabolic action and enzyme-inducing action of HC was discussed.
著者関連情報
© The Japan Endocrine Society
前の記事 次の記事
feedback
Top