抄録
The active leukocyte-increasing fractions prepared by tryptic digestion of parotin were separated by fractional precipitation with trichloroacetic acid, and two active peptides, parotin-T. B1 and parotin-T. B2 were isolated by the acid alumina column chromatography. Both peptides were estimated as to be homogeneous by the paper electrophoresis.Parotin-T. B1 showed the calcium activity and the leukocyte increasing response. Parotin-T. B2 showed only the leukocyte increasing response.
Some properties of parotin-T. B1 and parotin-T.B2 were described and also compared with those of parotin and parotin-T. A.
Parotin-T. B1 and parotin-T. B2 showed same ultraviolet absorption maximum at 275mμ of wave length. The amino acids components of both peptides were shown to be the same following 14 kinds; alanine, arginine, aspartic acid, glutamic acid, glycine, histidine, leucine (and/or isoleucine), lysine, proline, serine, threonine, tryptophan, tyrosine and valine. The N-terminal amino acid of these peptides were estimated as aspartic acid.
The activity of sensitization against parotin was recognized in parotin-T. A and parotin-T. B1 by the method using the uterus of guinea pig sensitized by parotin previously. But in parotin-T. B2 it was lacking.
