抄録
It has been more than 15 years since the advent of Next Generation Sequencing (NGS), which has made it possible to read DNA sequences much faster and at a lower cost than the Sanger method. A few years ago, Third Generation Sequencing (TGS) was also introduced to replace the short reads (only single fragments of 300 bp can be read) of Next Generation Sequencing with long reads (>100 Kbp can be sequenced at once). The pace of innovation in this field has accelerated rapidly over the last few years. This paper provides an overview of the applications of next generation sequencing and third generation sequencing in food microbiology testing as of 2021. It also provides a forecast of the impact of these new technologies on food microbiology testing in the near future. Due to space constraints, the third generation sequencers are discussed with particular emphasis on the molecular epidemiological analysis of foodborne illness caused by foodborne pathogens carried out by public authorities. In particular, third generation sequencing, especially of long reads, is expected to revolutionise the detection of foodborne pathogens, which has so far relied on culture and PCR methods. This will be of great help in determining the causes illness caused by foodborne pathogens. The analysis of microorganisms in food is currently undergoing rapid changes with NGS and TGS.
