抄録
The catalytic activities of metal-wool complexes for hydroxyl radical formation were compared with the corresponding metal-EDTA complexes in the presence of H202 (H202-added system) and in the presence of xanthine oxidase and hypoxanthine (XOD-HPX system) that produce H202. Hydroxyl radicals were formed from H202 via the metal-catalyzed Harber-Weiss reaction. In the H202-added system, Cu(II)-wool complex catalyzed the hydroxyl radical formation effectively, but Fe(III)-wool complex did not. To the contrary, Fe(III)-wool complex acted as a good catalyst in the XOD-HPX system. Superoxide anion radical was a very important species to reduce these transition metals judging from the enzymatic activities of superoxide dismutase and catalase. As hydroxyl radical scavengers, wool proteins inhibited the degradation of 2-deoxy-D-ribose, that is, the wool proteins captured hydroxyl radicals preferentially in competition with 2-deoxy-D-ribose.
