抄録
In order to elucidate the relation to in vivo lipid peroxidation and antioxidant defenses in cultured fish, in vivo lipid peroxidation levels and antioxidant activities in the liver and plasma of cultured yellowtail Seriola quinqueradiata obtained from Farm A and Farm B in Kagoshima Prefecture were compared with those of wild fish caught in the Yatsushiro Sea. The liver 2-thiobarbituric acid reactive substance (TBARS) values were significantly higher in Farm A fish and numerically higher in Farm B fish than in wild fish. Similarly the plasma TBARS values were significantly higher in Farm A fish and numerically higher in Farm B fish than in wild fish. The levels of α-tocopherol and ascorbic acid in the liver of Farm A fish were significantly lower than those of Farm B fish. This result may reflect the dietary levels of these vitamins. The levels of α-tocopherol and ascorbic acid in the liver of Farm A fish were also significantly lower than those of wild fish. Bilirubin was detected in the plasma of Farm A, suggesting an early stage of jaundice. Glutathione peroxidase activities in the liver and erythrocytes of cultured fish were significantly higher than those of wild fish. Superoxide dismutase activities in the liver of cultured fish were significantly higher than those of wild fish, but no difference was observed between the erythrocytes enzyme of cultured and wild fish. Aspartate and alanine aminotransferase activities in the plasma of cultured fish were significantly higher than those of wild fish. These results suggest that cultured fish may suffer from more oxidative stress than wild fish. Therefore, α-tocopherol and ascorbic acid supplements are necessary in the diets of yellowtail to prevent the progress of in vivo lipid peroxidation which may brings about many diseases.
