抄録
A cDNA clone encoding troponin-T of ezo-giant scallop Patinopecten yessoensis striated adductor muscle has been isolated and sequenced. The cDNA is composed of 1, 939 bp with a translational region of 975 bp at the nucleotide positions 111-1, 085. It deduced 325 amino acid residues with a molecular weight of 38, 438. Ezo-giant scallop troponin-T showed high homology with akazara scallop troponin-T (92%). However, it possesses 11 amino acid residues more than akazara scallop troponin-T and substitutions of 26 residues. According to sequence comparison with rabbit troponin-T, the residues 39-126 and 180-233 of ezo-giant scallop troponin-T appears to be tropomyosin-interacting regions and the residues 206-210 to be troponin-I-interacting region. Moreover, ezo-giant scallop troponin-T was assumed to have close phylogenetic relations to troponin-Ts of akazara scallop, nematode Caenorhabditis elegans, and fruit fly Drosophila melanogaster.
