2009 年 15 巻 5 号 p. 499-506
Major flavonoids contained in petals of edible chrysanthemum flowers (Chrysanthemum×morifolium Ramat. forma esculentum Makino, c.v Kotobuki) were identified, and their chemical compounds in relation to their radical scavenging activities and preventive effects against liver injury were compared. Based on retention times and UV spectra, three peaks on the HPLC chromatogram of the polyphenol fraction of edible chrysanthemum flowers confirmed the presence of luteolin 7-O-glucoside, apigenin 7-O-glucoside, and luteolin. Spectroscopic analysis determined the chemical structure of the three newly isolated compounds to be luteolin 7-O-(6''-O-malonyl)-glucoside, apigenin 7-O-(6''-O-malonyl)-glucoside, and acacetin 7-O-(6''-O-malonyl)-glucoside. Increases in plasma aspartate aminotransferase and alanine aminotransferase activities in mice (induced with liver injury by injection of carbon tetrachloride) were strongly suppressed by oral administration of luteolin and luteolin 7-O-(6''-O-malonyl)-glucoside, which have stronger radical scavenging activity than other compounds. Thus, it is suggested that compounds with chemical structures such as luteolin and luteoin 7-O-glucoside, which have maolonic acid on its glucosyl moiety, appear to readily available for mitigation of liver injury.
