Characterization of a Corrinoid Compound from Pacific Bluefin Tuna (Thunnus orientalis) Liver

Yukio ISHIHARA; Emi IMAI; Shigeo TAKENAKA; Tomoyuki FUJITA; Yukinori YABUTA; Shinya MASUDA; Fumio WATANABE

doi:10.3136/fstr.17.589

Notes

Characterization of a Corrinoid Compound from Pacific Bluefin Tuna (Thunnus orientalis) Liver

Yukio ISHIHARA, Emi IMAI, Shigeo TAKENAKA, Tomoyuki FUJITA, Yukinori YABUTA, Shinya MASUDA, Fumio WATANABE

著者情報

キーワード: pacific bluefin tuna, corrinoid compound, liver, Thunnus orientalis, vitamin B₁₂

ジャーナルオープンアクセス

2011 年 17 巻 6 号 p. 589-594

DOI https://doi.org/10.3136/fstr.17.589

詳細

抄録

A substantial amount of vitamin B₁₂ (357 ± 42.7 μg/100 g) was detected in pacific bluefin tuna (Thunnus orientalis) liver using a microbiological assay method. To evaluate whether tuna liver contains true vitamin B₁₂ or an inactive corrinoid compound, a corrinoid compound was purified and characterized. The purified compound was identical to that of authentic vitamin B₁₂, judging by TLC and LC/ESI-MS/MS chromatograms, and ¹H NMR spectral data. These results indicate that pacific bluefin tuna liver contains substantial amounts of true vitamin B₁₂ and would be an excellent source of the vitamin.

責任著者(Corresponding author)

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