抄録
The palI gene promotes plasma membrane localization of PalH in Aspergillus nidulans. We analyzed the role of palI in the ambient pH signal transduction pathway of A. oryzae by disrupting the palI gene. Polymerase chain reaction and Southern hybridization analyses indicated that homologous recombination occurred at the resident palI locus. palI disruption resulted in significantly decreased pacC expression and alkaline protease activity. Based on these results, we believe that palI plays an important role in the ambient pH signal transduction pathway of A. oryzae. While the palI-disrupted strain showed some growth on plates at alkaline pH, the palH-disrupted strain showed no growth. Thus, the results indicate that palI plays an important but not essential role in the pH signal transduction pathway of A. oryzae.
