Original papers
Anti-allergic Effects of the Alkaline Hydrolysis of Rapeseed Cake in a Rat Basophilic Leukemia Cell Line (RBL-2H3)
2018 年 24 巻 5 号 p. 935-942
詳細
2018 年 24 巻 5 号 p. 935-942
We investigated the anti-allergic activities of rapeseed cake extract (RCEx) using RBL-2H3 cells. RCEx was produced using the solvent methanol:acetone:water (7:7:6, v/v/v) and was also alkaline-hydrolyzed (AH-RCEx) using HP-20 column and the ethanol stepwise method. AH-RCEx showed a concentration-dependent decrease in degranulation in RBL-2H3 cells, whereas RCEx did not. The water-extracted fraction (WEx) from AH-RCEx mildly inhibited degranulation, and the 40% and 60% ethanol-eluted (EtEx) fractions of AH-RCEx significantly inhibited degranulation compared with that in the control. In HPLC and TLC, the main peak of WEx was consistent with sinapinic acid (SA). In addition, we found a characteristic m/z 473.15 peak in the 40% and 60% EtEx, which was not SA. Therefore, it was suggested that the anti-allergic constituents in rapeseed cake were successfully extracted by alkaline hydrolysis, and their activities were attributed to SA and other unknown compounds.
