Original papers
Purification of Endopeptidase in Yam Dioscorea opposita and Its Characterization
1999 年 5 巻 3 号 p. 259-261
詳細
1999 年 5 巻 3 号 p. 259-261
An endopeptidase in powdered yam was purified using a combination of anion-exchange and gel-filtration chromatographies. The preparation gave 9.2-fold purification with yield of 19% against the crude extract. The endopeptidase was classified as a serine endopeptidase with inhibitory spectrum, with an optimum temperature and pH of approximately 60℃ and 7.1, respectively. It also had high heat stability and high salt resistance.
