Anti-glycation and antioxidant effects of Chaga mushroom decoction extracted with a fermentation medium

抄録

Glycation reactions between proteins and sugars or their metabolites produce advanced glycation end-products, and the glycation of collagen in normal human dermal fibroblasts (NHDF) causes skin spots and freckles. UV exposure induces oxidative stress in NHDF, which overexpresses enzymes that degrade collagen, resulting in dry skin and wrinkling. Herein, we produced a novel polyphenolic decoction of Chaga mushroom extracted with a fermentation medium. Chaga polyphenol decoction (CPD) inhibited the glycation of albumin and collagen gel 3 to 4 times more than 2-aminoguanidine. The antioxidant effects of CPD were investigated using the fluorescence of an intracellular reactive oxygen species (ROS) scavenger, and NHDF exposed to UV-A for 60 min (9.5 J/cm2) after pre-treatment with 190 µg/mL of CPD suppressed ROS scavenger emission by 50 % compared to treatment with phosphate buffer saline. These results suggest that CPD might be a promising glycation inhibitor and ROS scavenger.