Estimated Levels of Xenoestrogens, Bisphenol A and 4-nonylphenol, in Human Breastmilk in Beijing Determined by High Performance Liquid Chromatography-Tandem Mass Spectrometry after Derivatization

抄録

To aid in the management of high-risk populations, bisphenol A (BPA) and 4-nonylphenol (4-NP) exposure from formula and human breastmilk was estimated in newborns and infants (aged 0-24 months) in Beijing. Derivatization with dansyl chloride coupled with high performance liquid chromatography-tandem mass spectrometry was performed to analyze traces of BPA and 4-NP in human breastmilk. Danyl-BPA and danyl-4-NP were easily obtained as a highly selective method, which was applied to 118 human breastmilk samples. The results indicate that newborns and infants (aged 0-24 months) are safe in terms of dietary exposure when fed by breastmilk, but are at higher exposure risk when fed by formula milk at 0-12 months of age. BPA and NP exposure from human breastmilk is under tolerable daily intake of 4 µg.kg^-1 bw.d^-1 and 5 µg.kg^-1 bw.d^-1, respectively. These results supplement the estimated exposure database for BPA and 4-NP in Beijing, especially for newborns and infants.