Effects of dietary supplementation with α-, β-, or γ-cyclodextrin on intestinal fermentation and composition of fecal microbiota in rats

抄録

Cyclodextrins (CDs) are cyclic oligosaccharides with attractive applications in the food industry. The most common CDs are α-CD, β-CD, and γ-CD, consisting of 6, 7, and 8 glucose units, respectively. This study aimed to compare the digestion, fermentation, and modulation of microbial composition among the 3 types of CDs in a rat model. Feeding of the diet containing 2.5 % (w/w) of α-CD or β-CD, but not γ-CD, for 2 weeks significantly increased the pool of succinate in the cecal content compared to the control. Feces collected during the first week of feeding contained α-CD and β-CD at detectable levels. Fecal microbial analysis revealed that α-CD and β-CD, but not γ-CD, showed an increasing trend in the relative abundance of the families Coriobacteriaceae and Bacteroidaceae, and decreased Clostridiaceae. Taken together, undigested α-CD and β-CD were utilized fermentatively with the production of succinate and lactate, particularly with changes in the microbial composition.