Abstract
With the advent of the CRISPR/Cas9 system, genome editing in various fields is advancing. Unintended mutation in off-target regions is a major problem of genome editing using the CRISPR/Cas9 system, and it is being reviewed. However, we found a high frequency and various unintended mutations in the “on-target” region when we generated a “knock-in” mouse with point mutation using this technique to develop a supernumerary rib model. Additionally, an inserted sequence of unknown origin was observed. Furthermore, these mutations were transferred to the next generation, even if tandem knock-in or large deletions occurred. These strongly suggest that a proper selection that meets the purpose is essential when considering the safety of foods and medicines using the genome-editing technology.
Vinagre, T., Moncaut, N., Carapuço, M., Nóvoa, A., Bom, J. and Mallo, M. (2010): Evidence for a myotomal Hox/Myf cascade governing nonautonomous control of rib specification within global vertebral domains. Dev. Cell, 18, 655-661.
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