Fundamental Toxicological Sciences
Online ISSN : 2189-115X
ISSN-L : 2189-115X
Original Article
Diverse unintended on-target mutations induced by zygote genome-editing using CRISPR/Cas9 system
Toshime IgarashiYukuto YasuhikoRyuichi OnoErika TachiharaMiki UchiyamaAtsuya TakagiYu TakahashiMakiko KuwagataSatoshi Kitajima
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2021 年 8 巻 5 号 p. 161-167

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With the advent of the CRISPR/Cas9 system, genome editing in various fields is advancing. Unintended mutation in off-target regions is a major problem of genome editing using the CRISPR/Cas9 system, and it is being reviewed. However, we found a high frequency and various unintended mutations in the “on-target” region when we generated a “knock-in” mouse with point mutation using this technique to develop a supernumerary rib model. Additionally, an inserted sequence of unknown origin was observed. Furthermore, these mutations were transferred to the next generation, even if tandem knock-in or large deletions occurred. These strongly suggest that a proper selection that meets the purpose is essential when considering the safety of foods and medicines using the genome-editing technology.

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© 2021 The Japanese Society of Toxicology
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