遺伝学雑誌
Online ISSN : 1880-5787
Print ISSN : 0021-504X
ISSN-L : 0021-504X
Molecular cloning of a Bacillus subtilis gene involved in cell division, sporulation, and exoenzyme secretion
Yoshito SADAIE
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ジャーナル フリー

1989 年 64 巻 2 号 p. 111-119

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The wild type div-341+ gene of Bacillus subtilis was cloned in a temperate phage ρ11, and was recloned in a smaller temperate phage φ105. The resulting Div+ transducing phage carried a 3 kilobase Cfr13I digested chromosomal fragment which showed Div+ transforming activity and contained the whole div-341+ gene which is involved in cell division, sporulation, exoenzyme secretion, competent cell formation, and autolysis. A partial restriction map of the fragment was established. The merodiploid system of the div-341+ gene, wild type gene on the phage genome and mutant gene on the chromosome, resulted in the suppression of mutant phenotypes and indicated that the wild type div-341+ gene is dominant over mutant gene.
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© The Genetics Society of Japan
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