抄録
This paper proposed that the method of micro- or semimicro- diffusion analysis could be appelied for the determination of hydrocyanic acid content of plants such as Sorghum or Sudan grass and to give a reliable result for its determination. The standard procedure of determination of hydrocyanic acid in plants as follows ; The outer compartment of a diffusion unit is placed with fresh sample cut into 3×3mm, and added with a few drops of chloroform and 1 to 2ml of 0.02% sulfuric acid. Alkali picrate solution, 1.0 to 2.0ml are pippetted into the central well of unit. The unit is tightly covered with using of slightly acidified gum arabic and glycerol mixture, and then incubated at about 20〜25℃, for 12〜20hrs. The differential optical density between alkali hydrocyanic acid picrate and alkali picrate is measured at 510mμ.
