日本国内で育成されたペレニアルライグラス四倍体品種におけるゲノムサイズの品種・個体間差異

抄録

Tetraploid perennial ryegrass (Lolium perenne) is utilized as palatable grazing grass in Japan. The genome size of perennial ryegrass cultivars that was bred in Japan have not been reported yet, we estimated the genome size of 6 Japanese cultivars and a breeding line by flow cytometry in this study. As the results, the average genome sizes of the cultivars were varied in small range from 5487 to 5575 Mbp/C, and there was no significant difference. However, large genome size difference among individuals, at least 660 Mbp/C between minimum and maximum individuals, was observed in each cultivar and a breeding line. Based on fluorescence in situ hybridization analysis, one of the reason why the large difference arose was considered to be the existence of aneuploid. Seed productivity is commonly affected by aneuploidy, flow cytometry could be useful tool to reject aneuploids in breeding program of perennial ryegrass.