Collagen biosynthesis in a periosteal cell pellet culture system.

Mari Akiyama; Masaaki Nakamura

doi:10.14832/gsjsdmd.2007f.0.208.0

抄録

The present study examines collagen biosynthesis in a bovine periosteal cell pellet culture system using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot analysis. Under ascorbic acid conditions, expression of both collagen type 1 and procollagen type 1 in the supernatants was observed. These results showed that procollagen type 1 was converted to collagen type 1 and that bone regeneration was dependent on vigorous collagen biosynthesis.

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