Evaluation of the Efficiency of Continuous Capture Chromatography

抄録

Multi-column chromatography (periodic counter-current chromatography, PCCC) with protein A affinity resins is used as an efficient monoclonal antibody (mAb) continuous capture process. However, the evaluation of the efficiency of PCCC process compared with repeated batch (or cyclic) operation (RBO) is still not standardized. In this study, we proposed two criteria for evaluating the capture process efficiency, productivity P and buffer consumption per load amount, B_C. These criteria were calculated for 2 column, 3 column and 4 column-PCCC and for RBO. For PCCC, P was proportional to mAb concentration C₀ and inversely proportional to the number of columns, n_C since the loaded amount of mAb M_L was fixed for the calculation. B_c did not depend on C₀ or n_C as M_L was the same. For RBO, B_C was larger by 20% at residence time (RT) = 4 minutes, and by 50% at RT = 2 minutes whereas P was similar to or slightly lower than that for 2C-PCCC at the same RT (4 minutes).