自発トリガーによるマイクロ血漿抽出機構の提案

抄録

In this paper, we report on the development of a microfluidic device, which extract plasma from low volume whole blood (10 µL) aiming integration onto microfluidic system for micro analytical systems. The device autonomously performs separation of blood cells and transfer of separated plasma by steady rotation. Based on the original autonomous fluidic control theory, we developed the principle of automatic timed injection of pressurizing medium into the separation chamber. This realize transferring of separated plasma from blood separation chamber to other place, after separation blood cells by centrifugal force. When we demonstrated by the poly-dimethylsiloxane (PDMS) microfluidic device, it was confirmed that automatic plasma extraction was successfully implemented as designed. The efficiency of separation was evaluated from concentrations of blood cells in samples before and after extraction, and the rate of blood cells removal was 98.5%.