Supported lipid bilayer (SLB) is one of artificial biomembrane systems for the studies of transportation reactions through biomembranes using solid devices and surface scientific methods. A key step in the SLB formation is transformation of spherical lipid vesicles to a planar membrane. We investigated stimulation methods for adsorbed lipid vesicles on a streptavidin-modified SiO2/Si substrate to induce tethered-type SLB. We describe the effects of osmotic stress, Ca2+ addition, and a freeze-thaw cycle on the adsorbed vesicles, and on the condition of t-SLB.
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