誘電泳動インピーダンス計測法を応用した熱損傷菌の代謝評価に関する基礎検討

抄録

Constant monitoring and immediate control of fermentation processes have been required for advanced quality preservation in food industry. In the present work, simple estimation of metabolic states for heat-injured Escherichia coli (E. coli) in a micro-cell was investigated using dielectrophoretic impedance measurement (DEPIM) method. Temporal change in the conductance between micro-gap (ΔG) was measured for various heat treatment temperatures. In addition, the dependence of enzyme activity, growth capacity and membrane situation for E. coli on heat treatment temperature was also analyzed with conventional biological methods. Consequently, a correlation between ΔG and those biological properties was obtained quantitatively. This result suggests that DEPIM method will be available for an effective monitoring technique for complex change in various biological states of microorganisms.