電界配向を用いた出芽酵母細胞の生死判別法

抄録

A rapid and easy viability assay of budding yeast cells using the electro-orientation (EO) phenomenon in an AC electric field was developed without any reagents. Saccharomyces cerevisiae cell suspension was simply sandwiched between two indium-tin-oxide plate electrodes. The EO directions of each cell indicated whether the cells were alive or dead at a narrow frequency band of 10-20 MHz. By observing through the transparent electrodes, the living cells were appeared as perfect circule shape when oriented parallel to the electric field line, while the dead cells were appeared as snowman and egg shapes when oriented perpendicular to the field.