A high-sensitive biochemical gas sensor (bio-sniffer) with skin gas cell and gas concentrator for monitoring of ethanol gas concentration emanated from human skin was constructed and demonstrated. This biosensor measured the concentration of ethanol according to the fluorescence intensity change of nicotinamide adenine dinucleotide (NADH), which was produced by an enzymatic reaction of alcohol dehydrogenase. By introducing a gas concentrator using liquid nitrogen, we constructed a highly sensitive system for skin gas measurements. In order to measure skin gas using the bio-sniffer, we designed a measurement system for detecting concentrated gases by connecting the sensor with a gas concentrator and introducing concentrated skin gas to the sensing surface. The monitoring of low concentration of skin ethanol gas was realized after drinking based on alcohol metabolism.
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