In this research, an enzyme electrode sensor with a two-layer bubble-removal flow channel that can be used in a skin microperfusion system was fabricated. The first layer of the channel is placed on enzyme electrodes. The flow channel is connected to the second layer which has an outlet. A hydrophobic porous membrane is placed on the channel wall between the two layers on the inlet side of the electrodes. Thus, bubbles can bypass the electrodes by taking a shortcut through the hydrophobic porous membrane into the second layer. Since bubble removal results from the pressure difference between the layers, this flow channel can be used in both positive- and negative-pressure systems. The flow channel height and the pore size in the hydrophobic porous membrane were selected by measuring pressure differences between the two layers using different pumping methods. The bubble removal performance was evaluated during microperfusion measurements on porcine skin. In addition, the effect of the bubble-removal flow channel on lactate concentration measurements using an enzyme electrode was evaluated.
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