Acute Toxicity of Textile Metal Complex Dark Green Azo Acid Dye (53) and Anionic Surfactant Oil on Nile Tilapia, Oreochromis niloticus and Bioconcentration of Total Chromium and Copper in Gill Tissues

抄録

The experiment was conducted on Nile tilapia Oreochromis niloticus to determine the lethal concentration of textile metal complex dark green azo acid dye in the presence and absence of anionic surfactant oil during an exposure period of 96 hr. The design consisted of four treatments: control, metal complex dark green azo dye, a mixture of metal complex dark green azo acid dye with anionic surfactant oil and anionic surfactant oil; and each was applied at four concentrations (1%, 5%, 10% and 15% of stock solution). Data were analyzed by using Probit analysis. The 96 hr LC50 was 7.25% (95% CL = 6.55-7.96) of stock solution for metal complex dark green azo acid dye, 2.85% (95% CL 2.14-3.90) for the mixture of metal complex dark green azo acid dye with anionic surfactant oil and 7.27% (95% CL = 6.45-8.43) stock anionic surfactant oil. Total chromium concentration in the gill filaments of tilapia increased linearly with dye concentration and was independent of anionic surfactant oil. Contrary, total copper concentration in the gill tissue increased linearly in with the present of anionic surfactant oil. The lethal effect observed during the trial was dose depended, the mortality rate increased with the increase in treatments concentration.