EFFECTS OF CADMIUM ON SOME ENZYME ACTIVITIES OF RAT LIVER-WITH SPECIAL REFERENCE TO DNA AND PROTEIN SYNTHESIS IN THE NUCLEI
1978 年 16 巻 2 号 p. 73-80
詳細
1978 年 16 巻 2 号 p. 73-80
Male Wistar rats were injected intraperitoneally with cadmium acetate (20μmoles/kg body weight) at two day intervals for six times. The increase of body weight was inhibited by the first injection of cadmium, but subsequent injections did not affect the further increase of body weight.
The weights of liver, kidney and spleen of the cadmium treated rats were not differed from control rats after six intraperitoneal injections. The contents of DNA and protein of livers were also not affected by the administration of cadmium.
The nuclear 3H-dTTP incorporating activity and the activity of glucose-6-phos-phatase of livers were inhibited by the treatment of cadmium, while 14C-leucine incorporating activity of nuclei, acid phosphatase and monoamine oxidase were hardly affected.
It is suggested that cadmium inhibits the DNA polymerase activity directly or by affecting synthesis of the enzyme, or affects the initiation sites on DNA chains. It is also suggested that the enzymes participated in the nuclear incorporation of amino acids are fairly resistant to cadmium. These results might be of value to help explain the carcinogenic or teratogenic action of cadmium.
