A molecular phylogenetic relationship of certain species of Argyranthemum found in the Canary Islands of Spain on the basis of the internal transcribed spacer (ITS)

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Seeds of eight species and one subspecies of Argyranthemum were experimentally collected, sown, germinated, cultivated and then, their ITS (Internal Transcribed Spacer) were molecularly studied to find interspecific relationships and phylogenetic trees of their relationships. The ITS base sequence of Argyranthemum callichrysum was found and described here for the first time. Argyranthemum frutescens subsp. succulentum and A. webbi were very closely related with each other concerning the phylogenetic trees of ITS base sequences. Additionally, A. callichrysum and A. broussonetii were also very closely related with each other concerning the phylogenetic trees of ITS base sequences. On the other hand, Glebionis coronaria (L.) P. Trehane was included in the clade of Argyranthemum species, according to their ITS sequences. Especially, G. coronaria was closely related with A. callichrysum than the other Argiranthemum species studied.