研究
ウサギ VX2 舌癌とその深頸リンパ節転移巣の増殖とアポトーシスの発現に関する病理学的研究
1998 年 23 巻 1 号 p. 1-15
詳細
1998 年 23 巻 1 号 p. 1-15
The proliferation kinetics of VX2 cancer cells transplanted to the tongue and its lymph node metastasis and the association of apoptosis were explored. On 7,14,21 and 28 days after VX2 cancer cells were transplanted into the left muscular layer of the tongue, rabbits were sacrificed to enucleate the tongue and deep cervical lyrnph node. Paraffin sections, prepared according to the ordinary procedure, were observed by hematoxylin-eosin staining, argyrophilic nucleolar organizer regions (AgNORs) staining and terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate-biotin nick end labeling (TUNEL) method.
The volume of rabbit tongue cancer increased rapidly 14 to 21 days after transplantation. The number of AgNORs in the peripheral portions of the rabbit tongue cancer cell nest was largest 14 days after transplantation. Metastasis was observed in a rabbit deep cervical lymph node on the transplantation side 14 days after transplantation. The number of AgNORs in the peripheral portions of the bilateral deep cervical lymph node metastasis was larger than that in the peripheral portions of the rabbit tongue cancer cell nest, increasing day by day with advance of the deep cervical lymph node metastasis. The apoptotic indexes of the rabbit tongue cancer and deep cervical lymph node metastasis increased day by day, then the latter exceeded the former. The number of AgNORs was correlated with the apoptotic index in the deep cervical lymph node metastasis on the transplantation side and in the rabbit tongue cancer cell nest.
Thus, the number of AgNORs in the rabbit VX2 tongue cancer cell nest, and its deep cervical lymph node metastasis, can be an indicator of proliferation activity of cancer cells. Especially, a rapid increase in the former may indicate deep cervical lymph node metastasis. It was suggested that apoptosis may be associated with the proliferation and advance of cancer cells in the rabbit tongue cancer cell nest and deep cervical lymph node metastasis.
